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MedCalc Software Ltd
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TURBOMOLE GmbH
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TissueGnostics
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TURBOMOLE GmbH
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CambridgeSoft Corporation
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Bioedit Company
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TURBOMOLE GmbH
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TissueGnostics
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Image Search Results
Journal: Cell reports
Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia
doi: 10.1016/j.celrep.2024.115056
Figure Lengend Snippet: WT ( Vim +/+ Foxp3 YFP-Cre ) and vimentin cKO ( Vim fl/fl Foxp3 YFP-Cre ) mice were intratracheally infected with lethal (60 plaque-forming units [PFUs]) (A) or a sublethal (30 PFUs) (A–K) dose of IAV. (A) Survival curve for WT ( n = 15, lethal; n = 9, sublethal) and vimentin cKO ( n = 9, lethal; n = 8, sublethal) mice using log rank (Mantel-Cox) test. (B) Percentage of weight loss from baseline in WT ( n = 8) and vimentin cKO ( n = 10) mice, analyzed by mixed-effects model with Sidak’s multiple comparisons test. Data are mean ± SEM. (C) Representative H&E lung staining at 21 dpi. Scale bar, 100 μm. (D) Lung injury score from H&E sections. (E) CD45 staining of lungs at 21 dpi. Scale bar, 100 μm. (F) Flow cytometry quantification of CD45 + cells from whole lung at 21 dpi. (G) Krt5 and Foxp3 staining of lungs at 21 dpi. Scale bars, 100 μm (bright field) and 50 μm (fluorescence). (H and I) Quantification of Krt5 expression (H) and frequency (I) using TissueGnostics HistoQuest. (J) IFN-β levels in BALF by multiplex immunoassay ( n = 3–8 mice per group). (K) Viral titer of whole-lung homogenates at 5 dpi. Data are mean ± SD, n = 4–6 mice per group. p values were calculated by two-way ANOVA (B and J) and two-tailed unpaired Student’s t test (D, F, H, I, and K). NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.005, and **** p < 0.0001.
Article Snippet:
Techniques: Infection, Staining, Flow Cytometry, Fluorescence, Expressing, Multiplex Assay, Two Tailed Test
Journal: Cell reports
Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia
doi: 10.1016/j.celrep.2024.115056
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Purification, Blocking Assay, Plasmid Preparation, Functional Assay, Control, Virus, Recombinant, Avidin-Biotin Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Software